limiting dilution中文什么意思

limiting: adj. 限制(性)的，有限制力的，起限定作用的〔不涉及 ...
dilution: n. 1.沖淡；稀釋。 2.稀度，淡度。 3.稀釋物。 ...
limiting dilution analysis: 有限稀釋分析:應(yīng)用波松分布來估計一群細(xì)胞中具有特定的特異性和功能的反應(yīng)細(xì)胞的頻率的方法
limiting dilution assay: 有限稀釋法
limiting dilution technique: 有限稀釋技術(shù)

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Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe . wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ) , and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
該純化cpe對小白鼠的半數(shù)致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起新西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。

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